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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/07/2020 |
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Actualizado : |
03/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
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Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
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Título : |
Application of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
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ISSN : |
0167-6857 |
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DOI : |
10.1007/s11240-020-01891-6 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
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Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
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Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02904naa a2200313 a 4500
001 1061249
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
022 $a0167-6857
024 7 $a10.1007/s11240-020-01891-6$2DOI
100 1 $aNARANCIO, R.
245 $aApplication of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.).$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020.
520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V.
653 $aAgrobacterium-mediated transformation
653 $aDelayed leaf senescence
653 $aMultigene
653 $aT-DNA
700 1 $aDING, Y.-L.
700 1 $aLIN, Y.-H.
700 1 $aSAHAB, S.
700 1 $aPANTER, S.
700 1 $aHAYES, M.
700 1 $aJOHN, U.
700 1 $aANDERSON, H.
700 1 $aMASON, J.
700 1 $aSPANGENBERG, G.
773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
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| 1. |  | CONDE, P.; VILLAMIL, J.J.; BIANCHI, D.; ZOPPOLO, R.; ENRIGHT, J.P.; SÁNCHEZ, A.; OLIVERA, A.; FRANCIA, B.; GONZÁLEZ, S. INIA y APPU juntos fortaleciendo el desarrollo del sector pecanero. Hortifruticultura. Revista INIA Uruguay, Junio 2023, no.73, p.52-55. (Revista INIA; 73).| Tipo: Artículos en Revistas Agropecuarias |
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